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Hi, wanted to know why when we use the chat history, the bot still has not context despite following the documentation properly:
json_data = { 'input_text': 'how to use it', 'chat_history': [ {"message": "what is EnGen Spy Cas9 HF1", "sent": True}, {"message": "'EnGen Spy Cas9 HF1 is a high-fidelity variant of the EnGen Spy Cas9 NLS protein from Streptococcus pyogenes. It is an RNA-guided endonuclease used for site-specific cleavage of double-stranded DNA. This variant has reduced non-specific DNA cleavage and contains a nuclear localization sequence (NLS) on both ends of the protein.", "sent": True, 'sources': [{'url': 'https://botsonic.s3.amazonaws.com/xxxx', 'content': 'CHUNK [1]: Biolabs provides reagents to support a broad variety of CRISPR/Cas9 genome editing approaches. From introduction of Cas9 and single guide RNA (sgRNA) on plasmids, to direct introduction of Cas9 ribonucleoprotein (RNP) and detection of edits using next generation sequencing or enzymatic mutation detection, NEB provides reagents that simplify and shorten genome editing workflows. EnGen Spy Cas9 HF1: for high-fidelity genome engineering by direct introduction of active nuclease complexes with reduced off-target cleavage EnGen® Spy Cas9 NLS, EnGen Lba Cas12a, EnGen Sau Cas9, EnGen Seq1 Cas9: for genome engineering by direct introduction of active nuclease complexes EnGen® sgRNA Synthesis Kit: for rapid generation of microgram quantities of custom sgRNA EnGen® Mutation Detection Kit: for robust determination of targeting efficiency EnGen® Spy Cas9 Nickase: programmable site-specific DNA nicking for genome editing and in vitro applications EnGen Spy dCas9 (SNAP-tag): Programmable DNA binding activity compatible with broad variety of SNAP-tag conjugates Choose Type: Subcategories Protocols', 'id': '5c60c3b2-c699-4312-ba09-c4cc1fb3a7b5'}, {'url': 'https://botsonic.s3.amazonaws.com/xxxxx', 'content': 'CHUNK [2]: mutations (N497A/R661A/Q695A/Q926A) reduces off-target cleavage Ideal for direct introduction of Cas9/sgRNA complexes Compatible with EnGen sgRNA Synthesis Kit, S. pyogenes (NEB #E3322S) and the EnGen Mutation Detection Kit (NEB #E3321S) Reagents Supplied Featured Video + Bacterial Adaptive Immunity with CRISPR/Cas9 Catalog # Concentration Size M0667M 20 µM 2,500 pmol M0667T 20 µM 500 pmol To find out how to order this product from your current location, click the button below: International Customer & Order Support Please enter a quantity for at least one size Need a custom/large volume order? Contact us Product Information EnGen Spy Cas9 HF1 is a high-fidelity, quadruple substitution (N497A/R661A/Q695A/Q926A) variant of EnGen Spy Cas9 NLS from Streptococcus pyogenes with reduced non-specific DNA cleavage. Spy Cas9 is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The single guide RNA (sgRNA) targets Cas9 to the region immediately upstream of a 5′-NGG-3′ protospacer adjacent motif (PAM) producing a double stranded break 3 bases upstream of', 'id': '7d921e22-850b-412c-ad08-90b0800807c4'}, {'url': 'https://botsonic.s3.amazonaws.com/xxx', 'content': 'CHUNK [3]: a 5′-NGG-3′ protospacer adjacent motif (PAM) producing a double stranded break 3 bases upstream of the PAM (1). EnGen Spy Cas9 HF1 contains Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C-termini of the protein. Figure 1. Schematic representation of S. pyogenes Cas9 nuclease complexed with a single guide RNA and target DNA S. pyogenes Cas9 (Spy Cas9) protein is shown in beige, single guide RNA (sgRNA) is depicted in blue, and the DNA is shown in grey, green, and red. The DNA target, or protospacer, is shown in green and the base pairing complementarity of the target strand with the guide RNA in the R-loop is illustrated. The protospacer adjacent motif (PAM) which is required for Cas9 binding to DNA is shown in red and the locations of DNA strand cleavage are indicated with black triangles. Orange labels highlight the relative positions of mutated amino acid residues in Spy Cas9 HF1 (2) which contact the DNA backbone of the target strand in the PAM distal region of the RNP-DNA complex. Figure 2. EnGen Spy Cas9 HF1 demonstrates increased', 'id': '7d921e22-850b-412c-ad08-90b0800807c4'}]}, ] }